Search results for "Microsatellite enrichment"

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Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera…

2008

We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy–Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.

Geneticseducation.field_of_studyPopulationLocus (genetics)Biologybiology.organism_classificationNull alleleLoss of heterozygosityMayflyGeneticsMicrosatelliteeducationTrinucleotide repeat expansionMicrosatellite enrichmentEcology Evolution Behavior and SystematicsBiotechnologyMolecular ecology resources
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Isolation and characterization of 11 polymorphic trinucleotide microsatellite markers in the stonefly Arcynopteryx compacta (Plecoptera: Perlodidae).

2011

We describe the isolation of 11 polymorphic trinucleotide microsatellite loci from the stonefly Arcynopteryx compacta. Loci were highly variable with 3 to 14 alleles (mean = 6.45). Observed heterozygosity ranged from 0 to 0.867. Seven loci showed significant deviation from Hardy–Weinberg equilibrium across both populations. There was no evidence for null alleles, and thus, Hardy–Weinberg departures could have resulted from genetic structure between populations or subpopulations. No linkage between loci was found. The 11 loci should prove highly informative for population genetic studies.

Geneticseducation.field_of_studybiologyPopulationbiology.organism_classificationNull alleleLoss of heterozygosityPerlodidaeGenetic structureGeneticsMicrosatelliteAlleleeducationMicrosatellite enrichmentEcology Evolution Behavior and SystematicsBiotechnologyMolecular ecology resources
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